HIV ECLIA

Originally Posted: 
Monday, August 13, 2012
Question: 

Hello,

I had an accident this summer with a patient when giving wound treatment. A curette penetrated latex glove that I was wearing and caused a small injury to my index finger. It never bled and I cleaned it immediately.  The patient was tested for HIV and hepatitis 3 months ago and tests were negative and no risk behavior in general.

However I want to be sure and was wondering how accurate this HIV ECLIA test would be after 5 weeks of contamination?

Answer: 

Hi there and thank you for using AIDS Vancouver as your source of HIV/AIDS related information. 

The HIV ECLIA (Electrochemiluminescence immunoassay) test is a 3rd Generation test that looks for antibodies in the blood. The World Health Organization and international guidelines consider HIV antibody tests definitive and completely accurate at 12 weeks (3 months) post exposure. That being said, 95% of infections are detectable with 4-6 weeks and most people develop antibodies in 21-25 days (approximately 3 weeks). Therefore, a test at 5 weeks is a good indicator of your status, however, a follow-up test at 12 weeks would be needed for completely conclusive results.

It is also important to note that one needs to be in contact with HIV+ fluids in order for HIV to be transmitted. If the patient was tested for HIV 3 months ago and it was a negative result, then unless they acquired HIV very recently (ie. within the past 3 months), there is no possibility of acquiring HIV.

I hope this helps answer your question, and if you have any additional questions please feel free to contact us again.

Take care,

Stephanie

AIDS Vancouver Helpline Volunteer

E-mail: helpline@aidsvancouver.org

Phone (Mon-Fri 9am-4pm): (604) 696-4666

Web: www.aidsvancouver.org/helpline

 

 

Comments

Submitted by Anonymous (not verified) on

Thank you for your answer,

I got negative result from the test today, which gave me a bit comfort. I forgot to mention that this ECLIA detects also p24 antigen so perhaps is gives it a bit more reliability than just an antibody test?

helpline2's picture
Submitted by helpline2 on

Hi again, 

Glad to hear about your results. The p24 antigen is present on the surface of the HIV virus, and detection of this protein allows for earlier diagnosis, since it is independent from antibody production. However, since antibodies bind to this antigen once produced, it is only detectable in the first few weeks of infection. This is why testing methods that use p24 antigen detection also include antibody based testing methods to allow for a continuum of HIV detection. Most HIV specialists consider negative test results obtained with these types of tests (termed 4th generation, AG/AB) conclusive at 6 weeks. While not necessarily more reliable (antigen testing alone is extremely reliable) adding p24 detection does significantly increase the probability of early infection detection. 

All the best, 

Jonathan
AIDS Vancouver Helpline Volunteer

Submitted by Teo (not verified) on

Hi,
I had a unprotected sex about 7 weeks ago , now I have had ECLIA test HIV 1 & 2 ab/ag, and the result is “not detected”. I wanna know that 95% accuracy is just within 4-6 weeks or after 6 weeks also the accuracy of test is high. TQ

Helpline1's picture
Submitted by Helpline1 on

Hi there and thanks for using the AIDS Vancouver Helpline as a source for HIV/AIDS related information.

The test you took at 7 weeks is commonly known as the 4th Generation EIA (ab/ag) Test.  The antigen (ag) portion of the test is detectable immediately after infection (3-4 days post-exposure) for the first 2-4 weeks.  The antibody (ab) portion of the test is detectable from 3-4 weeks onwards.  Many HIV Specialists consider this test conclusive at 6 weeks, however the World Health Organization (WHO) Guidelines consider all tests conclusive at 84 days or 3 months.

So, in answer to your question, your test at 7 weeks is a very good sign that you are negative and it would be very rare for those results to change at 3 months.  However, for your own peace of mind you may wish to be retested at 3 months.

I trust I have addressed your concerns however, if you have any further questions, please feel free to contact us again.

In Health,
Jon,
AIDS Vancouver Helpline Volunteer
Phone:  604.696.4666

Submitted by torron (not verified) on

i have made hiv test after 1 year possible expore..the test eclia method (hiv 1/2 ag/ab) not detected..is test very conclusive or not..

helpline2's picture
Submitted by helpline2 on

 

Hi there,

Thank you for using the AIDS Vancouver Online Helpline as your source of HIV-related information.

The World Health Organization considers all testing at or beyond three months post-exposure to be conclusive – your test at one year should be absolutely conclusive. You do not need to worry – the test was negative and you do not have HIV.

Stay safe,
Griffin
AIDS Vancouver Helpline Volunteer 

Submitted by Jay Kumar (not verified) on

Hi i had protected sex with lady in red light district amsterdam and after 22 days i had HIV 1 & 2 eclia test and result was not detictive 0.16. so what is that means and is there any risk
thank you
Kummar

Helpline1's picture
Submitted by Helpline1 on

Hi Kummar,

It is great to hear that you used a condom during sex; it is still considered the best way to protect yourself against HIV and other STI's.

Your negative antibody test at 22 days is a good indication of your HIV status (most people develop antibodies in 21-25 days). However, International testing guidelines still recommend you go for a re-test at 3 months for completely conclusive results. 

 

All the Best,

Elyse

AIDS Vancouver helpline

helpline@aidsvancouver.org

604 696 4666

Submitted by sandeep (not verified) on

result is non reactive after 13 days.
what is the accuracy of the test?

Helpline1's picture
Submitted by Helpline1 on

Hello there and thank you for using the AIDS Vancouver Helpline as a source of online HIV/AIDS related information.

Your test result at 13 days is a good indicator of your status.

As I might have read previous posts, ECLIA is also known as 4th generation EIA test (sometimes called Duo/Combo test). This test looks for 2 things: P24 antigen and antibodies.
While P24 is detectable immediately after infection and only for the first few weeks, up to 95% of infections are detectable within 4-6 weeks with antibody testing. For this reason, many HIV Specialists suggest 6 weeks as conclusive with this test. However, because we have to follow the local (BC Centre for Disease Control and Prevention) guideline, I still suggest you to retest at 12 weeks (3 months) to receive a conclusive result.

Hopefully you find the information helpful.
If you have any other questions/concerns, please feel free to write us back.

Stay healthy and keep smiling,
Yoshiko
AIDS Vancouver Helpline Volunteer

Submitted by Felix (not verified) on

Hi

I kissed a sex worker on Feb 15th and I regretted that and have been worried ever since. I love my girlfriend and my family so much and I dont wanna take any chance screwing up my life any more.

I took a HIV test in Germany on April 30th, 10 weeks after the risky action. The name of the test was "HIV 1/2 Ag/Ak", Result is negative, 0.14 s/co, reference value 1.0 . Could anyone explain to me what this "HIV 1/2 Ag/Ak" Test is and how accurate it is after 10 weeks? Is this test different from the Ag/Ab Test?

Thank you in advance!

helpline2's picture
Submitted by helpline2 on

Hi there,

Thank you for using the AIDS Vancouver Online Helpline as your source of HIV-related information.

I'd like to start by noting that it is not the person you engage with or their profession, but the activity that you engage in that poses the risk of HIV infection. Engaging with a sex worker puts you at no higher risk than does engaging with anyone else.

Kissing is absolutely a no-risk activity. You cannot transmit HIV through saliva, and so there is no risk of HIV transmission associated with kissing. The only activities that pose a risk of HIV transmission are sharing needles, and unprotected anal or vaginal sex.

Given that you did not have an exposure, you can trust your test results to confirm that you are HIV-negative. I've checked with my supervisor and as far as I can tell, the Ag/Ak test you refer to is the same as the Ag/Ab test -- most experts consider the duo test to be conclusive at six weeks.

I hope I've been able to answer your questions -- please let us know if you have any further concerns.

Best wishes,
Griffin
AIDS Vancouver Helpline Volunteer

Helpline1's picture
Submitted by Helpline1 on

Hello and thank you for using the AIDS Vancouver helpline as a source of HIV related information.

Non-reactive means negative. Index value is not important; however, it is important to look whether a result is "reactive" (positive) or "non-reactive" (negative).

3 months is considered conclusive by the World Health Organization and the international guidelines for HIV testing; thus your test result at 5 months is conclusive and definite; you are HIV negative. No further test is necessary as long as you have not engaged in any high risk activities (unprotected anal/vaginal sex, sharing needles) during this 5 months.

Hopefully you find the information helpful.
if you have any other questions, please feel free to write us back.

Stay healthy and keep smiling,
Yoshiko
AIDS Vancouver Helpline Volunteer

Submitted by Rahul (not verified) on

Hi,

I had an unprotected sexual encounter with a colleague in March 2013. Since she was on pills, she insisted that we have unprotected sex and and both of us were a little drunk, I did not think about the consequences that time though I regret it badly now

Now I am planning for a kid with my wife and hence have just given my HIV Test (using ECLIA) and I am awaiting the results.

As I was going through this forum i understand that there can be virus other than HIV like human papillomavirus (HPV), herpes simplex virus (HSV), and hepatitis B virus (HBV).

What are the recommended tests that I should do for the other virus and other STI.

Kindly help.

Thank You

Submitted by davidn on

Hello and thanks for the questions. As you are having unprotected sex with more than one person it is recommended that you test for all sexually transmitted infections.

No person can make you have unprotected intercourse and we always recommend the use of condoms  to prevent the passing of STI, including HIV.

For more information on all other sexually transmitted infections, please see our friends at www.smartsexresource.com

David

Submitted by Anonymous (not verified) on

Hello,
I would like to ask you a question that is really trouble me for almost 2 months.
I had as you can say hugging with a sex worker we didn't had sexual encounter, but I just touch her breasts and kiss her breasts for 4-5 minutes. The thing that is bordering me is that after I found out that I have small cut on my lower lip on a left corner of it (not so open (maybe 1,5 mm wide and 2-3 mm long ) and not so exposed when my lips were closed) and when I kissed her 1-2 times on her breasts she smelled like male seamen (her skin was dry there was no liquid of any kind but still smelled like semen) - that got me worried - it got me thinking - what if someone in very close time before had a sexual encounter with her and in fact some of his semen got on her breasts?
Had that said I got ECLIA test (HIV Combo Anti 1+2 i p24 Ag (ECLIA)) on 41'st day after exposure and it came out negative.
So my question would be: was that encounter exposure to HIV and if it was should I consider taking another test after 3 months?
Thanks in advance for your answer.

Submitted by davidn on

Hello the act of kissing a breast will not pass the HIV virus and there is no reason to continue testing if this was your only sexual activity. The high risk activities that may pass HIV are unprotected vaginal and anal intercourse and injecting with shared needles.

Taking breast milk into the mouth is only a negligible risk for HIV infection and we have no evidence that there has ever been HIV transmission to an adult from this act.

When we talk about breast milk passing the HIV virus, we are refering to children of HIV positive mothers that are getting their only food supply from this source.

An HIV 4th Generation or Combo test is highly accurate and reliable and many HIV specialists will consider this test to be conclusive at just 6 weeks.

We can spend our entire lives thinking what if, however we at the Helpline only refer to factual information and not what if situations. 

David


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